- Cancer & ozone
- Ozone & Neoplastic growth
- Viral diseases
- Anti-inflammatory effects
- Ozone & red blood cells
- Tumor oxygenation
- Ozone therapy in head/neck tumors
- RADIATION induced rectal bleeding
- Clinical review
- ozone & oxidative stress
- OZONE inhibits cancer growth
Ozone Therapy in Cancer Treatment: State of the Art
Silvia Mene´ndez, Janet Cepero, and Luis Borrego
Erhlich Ascitic Tumor and Sarcoma 37 were implantedin mice and afterward the animals were treated with ozone (rectally). A significant decrease in the number of metas- tasis was obtained. In another study, ozone was applied intraperitoneally, before Lewis’ lung carcinoma inoculation. A delayed effect in the tumor development kinetics and in the increase rate of tumor volume in the ozone groups was observed. With regard to the clinical trial, patients with prostatic cancer were treated with cobalt-60 therapy and ozone (rectally), decreasing the presence of side effects (due to radiation treatment) and the prostatic specific antigen figures. However, further investigations are neces- sary to be performed, in order to be considered the ozone therapy as complementary therapy for cancer.
Cepero, J, Borrego, L & Mene, S 2008, ‘Ozone Therapy in Cancer Treatment : State of the Art’, no. December, pp. 398–404.
Restoration of Normoxia by Ozone Therapy May Control Neoplastic Growth: A Review and a Working Hypothesis
VELIO BOCCI, M.D., Ph.D., ALESSANDRA LARINI, Ph.D., and VANNA MICHELI, Ph.D.
In contrast to normal tissues, tumors thrive in hypoxic environments. This appears to be because they can metastasize and secrete angiopoietins for enhancing neoangiogenesis and further tumor spread. Thus, during chronic ischemia, normal tissues tend to die, while neoplasms tend to grow. During the past two decades, it has been shown in arteriopathic patients that ozonated autohemotherapy is therapeutically useful because it in- creases oxygen delivery in hypoxic tissues, leading to normoxia. Although several oxygenation approaches have been tested, none is able to restore normoxia permanently in patients with cancer. We postulate that a prolonged cycle of ozonated autohemotherapy may correct tumor hypoxia, lead to less aggressive tumor behavior, and represent a valid adjuvant during or after chemo- or radiotherapy. Moreover, it may re-equilibrate the chronic oxidative stress and reduce fatigue.
Bocci, V, Ph, D, Larini, A, Ph, D, Micheli, V & Ph, D 2005, ‘Restoration of Normoxia by Ozone Therapy May Control Neoplastic Growth : A Review and a Working Hypothesis’, vol. 11, no. 2, pp. 257–265.
Ozonization of Blood for the Therapy of Viral Diseases and Immunodeficiencies.
Institute of General Physiology and Nutritional Sciences, University of Siena, 53100, Italy
In the last 3 decades major autohemotherapy after exposure to ozone has been used in Europe in uncontrolled trials carried out in patients with many illnesses, particularly chronic viral diseases and neoplasms. It appears that the treatment may activate the hosts immune system by inducing the production of immunoactive cytokines and it may now be possible to rationalize the procedure, improve the regimen and assess the outcome. It is apparent, however, that such a therapeutic approach, in order to be acceptable, requires an investigative effort of biologists and clinicians. Once this is done, owing to the large range of medical applications and the simplicity of the procedure, autohemotherapy could become very valuable particularly in underdeveloped countries.
Bocci, V n.d., ‘Ozonization of Blood for the Therapy of Viral Diseases and Immunodeficiencies . A Hypothesis’, pp. 30–34.
Anti‑inflammatory effects of ozonated water in an experimental mouse model
KAZUO AZUMA, TAKURO MORI, KINYA KAWAMOTO, KOHEI KURODA, TAKESHI TSUKA,TOMOHIRO IMAGAWA, TOMOHIRO OSAKI, FUMIO ITOH, SABURO MINAMI and YOSHIHARU OKAMOTO
Previous studies have suggested that ozonated water is safe and possesses antibacterial effects for treatment of experimental peritonitis rats. In this study, we evaluated the anti‑inflammatory effects of ozonated water that was intraper- itoneally injected into an experimental inflammatory mouse model. The concentrations of dissolved ozone decreased constantly and lineally, while the half-life of dissolved ozone was 36.8±2.7 min (27˚C). The 10‑ppm ozonated water was injected intraperitoneally into mice with lipopolysaccharide (LPS)‑induced acute inflammation. The results showed that the intraperitoneal injection of ozonated water decreased the levels of tumor necrosis factor-α (TNF-α) and increased the activity of superoxide dismutase (SOD). The results suggest that ozonated water has anti‑inflammatory properties and is a potential therapeutic option for acute inflammation.
Azuma, K, Mori, T, Kawamoto, K, Kuroda, K, Tsuka, T, Imagawa, T, Osaki, T, Itoh, F, Minami, S & Okamoto, Y 2014, ‘Anti ‑ inflammatory effects of ozonated water in an experimental mouse model’, pp. 671–674.
The effects of colorectally insufflated oxygen-ozone on red blood cell rheology in rabbits
A. Seda Artis, Sami Aydogan and M. Gokhan Sahin
Department of Physiology, Faculty of Medicine, University of Erciyes, Kayseri, Turkey
Currently, with reappraisal of ozone therapy, it has been utilized worldwide in research and clinical field. Most of the studies investigating effects of ozone on blood parameters are conducted by directly ozonating the blood. Rectal insufflation is a simple, easy and inexpensive method of delivering ozone. Little is known how these gases affect some fundamental hemorheologic parameters when given by insufflation. We aimed to investigate the effects of colorectally insufflated oxygen- ozone on red blood cell rheology in rabbits. Rabbits were divided into Group 1 (control); Groups 2, 3 and 4 (oxygen rectally insufflated respectively for 15, 21 and 36 days); Groups 5, 6 and 7 (ozone rectally insufflated respectively for 15, 21 and 36 days). Erythrocyte deformability, aggregation and osmotic fragility were determined from blood samples at the end of each treatment period. Our study showed an improvement in deformability, a decrease in aggregation and an increase in fragility following a 15 day ozone treatment.With longer ozone application the changes in aggregation and fragility returned back to control levels, however its effect on deformability sustained. Therefore, more than two weeks ozone insufflation may induce adaptation to changes induced by ozone suggesting its systemic effects.
Artis, AS, Aydogan, S & Sahin, MG 2010, ‘The effects of colorectally insufflated oxygen-ozone on red blood cell rheology in rabbits’, vol. 45, pp. 329–336.
Ozone Therapy for Tumor Oxygenation: a Pilot Study
Bernardino Clavo, Juan L. Pérez, Laura López, Gerardo Suárez, Marta Lloret, Victor Rodríguez, David Macías, Maite Santana, María A. Hernández, Roberto Martín-Oliva and Francisco Robaina
Tumor hypoxia is an adverse factor for chemotherapy and radiotherapy. Ozone therapy is a non- conventional form of medicine that has been used successfully in the treatment of ischemic disorders. This prospective study was designed to assess the effect of ozone therapy on tumor oxygenation. Eighteen subjects were recruited for the study. Systemic ozone therapy was administered by auto- hemotransfusion on three alternate days over one week. Tumor oxygenation levels were measured using polarographic needle probes before and after the first and the third ozone therapy session. Overall, no statistically significant change was observed in the tumor oxygenation in the 18 patients. However, a significant decrease was observed in hypoxic values ≤10 and ≤5 mmHg of pO2. When individually assessed, a significant and inverse non-linear correlation was observed between increase in oxygenation and the initial tumor pO2 values at each measuring time-point, thus indicating that the more poorly-oxygenated tumors benefited most (rho = –0.725; P = 0.001). Additionally, the effect of ozone therapy was found to be lower in patients with higher hemoglobin concentrations (rho = –0.531; P < 0.034). Despite being administered over a very short period, ozone therapy im- proved oxygenation in the most hypoxic tumors. Ozone therapy as adjuvant in chemo-radiotherapy warrants further research.Keywords:
Clavo, B, Pérez, JL, López, L, Suárez, G, Lloret, M, Rodríguez, V, Santana, M, Hernández, MA, Martín-oliva, R & Robaina, F 2004, ‘Ozone Therapy for Tumor Oxygenation : a Pilot Study’, vol. 1, no. 1, pp. 93–98.
Adjuvant Ozonetherapy in Advanced Head and Neck Tumors: A Comparative Study
Bernardino Clavo, Ana Ruiz, Marta Lloret, Laura López, Gerardo Suárez, David Macías, Victor Rodríguez, Maria A. Hernández, Roberto Martín-Oliva, Santiago Quintero, José M. Cuyás and Francisco Robaina
Advanced head and neck (H&N) tumors have a poor prognosis, and this is worsened by the occurrence of hypoxia and ischemia in the tumors. Ozonetherapy has proved useful in the treatment of ischemic syndromes, and several studies have described a potential increase of oxygenation in tissues and tumors. The aim of this prospective study was to evaluate the clinical effect of ozonetherapy in patients with advanced H&N cancer in the course of their scheduled radiotherapy. Over a period of 3 years, 19 patients with advanced H&N tumors who were undergoing treatment in our department with non- standard fractionated radiotherapy plus oral tegafur. A group of 12 patients was additionally treated with intravenous chemotherapy before and/or during radiotherapy. In the other group of seven patients, sys- temic ozonetherapy was administered twice weekly during radiotherapy. The ozonetherapy group was older (64 versus 54 years old, P?0.006), with a higher percentage of lymph node involvement (71% versus 8%, P?0.019) and with a trend to more unfavorable tumor stage (57% versus 8% IVb ?IVc stages, P?0.073). However, there was no significant difference in overall survival between the chemotherapy (median 6 months) and ozonetherapy (8 months) groups. Although these results have to be viewed with caution because of the limited number of patients, they suggest that ozonetherapy could have had some positive effect during the treatment of our patients with advanced H&N tumors. The adjuvant administration of ozonetherapy during the chemo–radiotherapy for these tumors merits further research.
Ozone Therapy in the Management of Persistent Radiation-Induced Rectal Bleeding in Prostate Cancer Patients
Bernardino Clavo, Norberto Santana-Rodriguez, Pedro Llontop, Dominga Gutierrez, Daniel Ceballos, Charlin Méndez, Gloria Rovira, Gerardo Suarez, Dolores Rey-Baltar, LauraGarcia-Cabrera, Gregorio Martínez-Sánchez, and Dolores Fiuza
Introduction. Persistent radiation-induced proctitis and rectal bleeding are debilitating complications with limited therapeutic options.We present our experience with ozone therapy in the management of such refractory rectal bleeding. Methods. Patients (푛=12) previously irradiated for prostate cancer with persistent or severe rectal bleeding without response to conventional treatment were enrolled to receive ozone therapy via rectal insufflations and/or topical application of ozonized-oil. Ten (83%) patients had Grade 3 or Grade 4 toxicity.Median follow-up aſter ozone therapy was 104months (range: 52–119). Results.Following ozone therapy, the median grade of toxicity improved from 3 to 1 (푝 < 0.001) and the number of endoscopy treatments from 37 to 4 (푝 = 0.032). Hemoglobin levels changed from 11.1 (7–14) g/dL to 13 (10–15) g/dL, before and aſter ozone therapy, respectively (푝 = 0.008). Ozone therapy was well tolerated and no adverse effects were noted, except soſt and temporary flatulence for some hours aſter each session. Conclusions. Ozone therapy was effective in radiation-induced rectal bleeding in prostate cancer patients without serious adverse events. It proved useful in themanagement of rectal bleeding and merits further evaluation.1.
Ozone therapy: A clinical review
A. M. Elvis and J. S. Ekta
Ozone (O ) gas discovered in the mid-nineteenth century is a molecule consisting of three atoms of3oxygen in a dynamically unstable structure due to the presence of mesomeric states. Although O has3dangerous effects, yet researchers believe it has many therapeutic effects. Ozone therapy has been utilized and heavily studied for more than a century. Its effects are proven, consistent, safe and with minimal and preventable side effects. Medical O is used to disinfect and treat disease. Mechanism of actions is by3inactivation of bacteria, viruses, fungi, yeast and protozoa, stimulation of oxygen metabolism, activation of the immune system. Medication forms in a gaseous state are somewhat unusual, and it is for this reason that special application techniques have had to be developed for the safe use of O . In local applications as3in the treatment of external wounds, its application in the form of a transcutaneous O gas bath has3established itself as being the most practical and useful method, for example at low (sub-atmospheric) pressure in a closed system guaranteeing no escape of O into the surrounding air. Ozonized water, whose3use is particularly known in dental medicine, is optimally applied as a spray or compress. Diseases treated are infected wounds, circulatory disorders, geriatric conditions, macular degeneration, viral diseases, rheumatism/arthritis, cancer, SARS and AIDS.
Elvis, AM & Ekta, JS 2011, ‘Ozone therapy : A clinical review’, vol. 2, no. 1, pp. 66–70.
Effect of ozone/oxygen mixture on systemic oxidative stress and organic damage
Dailen Guanche, Zullyt Zamora , Frank Hernández, Karel Mena, Yaima Alonso, Maydelis Roda, Maritza Gonzáles, and Ricardo Gonzales
Ozone is a molecule of high energetic content. Its great oxidative power has been used in medicine for the treat- ment of several illnesses with a wide spectrum. The rectal insufflation with a mixture of ozone/oxygen is considered as a simple therapy, not painful, of low cost and practically free from adverse effects. Given its potential oxidation and lack of side-effects, the objective has been to know the state of different indexes of redox state in blood which may contribute to understanding the mechanism by which mixtures of ozone/oxygen administered by intrarectal route are able to exert actions on other organs. With this purpose female rabbits were used, distributed into four groups, and three doses of ozone/oxygen mixture were tested. When treatment was finished, the determination of pro-oxidant and antioxidant markers was carried out. Also indexes of organic damage were determined. Ozone doses administered to rabbits did not cause adverse effects and mortality did not show significant changes rela- tive to tissue damages and they increased enzymes activities belonging to the first line antioxidant defences. The results demonstrate that ozone/oxygen mixture administered by rectal insufflations is innocuous and it is able to increase the antioxidant defense of the organism.
Guanche, D, Zamora, Z, Hernández, F, Mena, K, Alonso, Y, Roda, M, Gonzáles, M & Gonzales, R 2010, ‘Effect of ozone / oxygen mixture on systemic oxidative stress and organic damage’, vol. 20, no. November 2009, pp. 25–30.
Ozone Selectively Inhibits Growth ofHuman Cancer Cells
WILLIAM A. HASELTINE KWOK MING LoALAN D. D'ANDREA
The growth ofhuman cancer cellsfrom lung, breast, and uterine tumorswas selectively inhibited in a dose-dependent manner by ozone at 0.3 to 0.8 part per million of ozone in ambient air during 8 days of culture. Human lung diploidfibro- blasts served as noncancerous control cells. The presence ofozone at 0.3 to 0.5 part per million inhibited cancer cell growth 40 and 60 percent,. respectively. The non- cancerous lung cells were unaffected at these levels. Exposure to ozone at 0.8 part per million inhibited cancer cell growth more than 90 percent and control cell growth less than 50 percent. Evidently, the mechanisms for defense against ozone damage are impaired in human cancer cells.
Meehan, T, Straub, K, Calvin, M & Lon-, N 1979, ‘Ozone Selectively Inhibits Growth of Human Cancer Cells’, vol. 79, no. 1975, pp. 2–4.